Publications EDyP-Service 2019-2024

2024

238648 24RQUPQD 2024 1 apa 50 date asc 1590 https://www.edyp.fr/web/wp-content/plugins/zotpress/
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Casanova, A. G., Roth, G. S., Hausmann, S., Lu, X., Bischoff, L. J. M., Froeliger, E. M., Belmudes, L., Bourova-Flin, E., Flores, N. M., Benitez, A. M., Chasan, T., Caporicci, M., Vayr, J., Blanchet, S., Ielasi, F., Rousseaux, S., Hainaut, P., Gozani, O., Le Romancer, M., … Reynoird, N. (2024). Cytoskeleton remodeling induced by SMYD2 methyltransferase drives breast cancer metastasis. Cell Discovery, 10(1), 1–22. https://doi.org/10.1038/s41421-023-00644-x
Janet-Maitre, M., Job, V., Bour, M., Robert-Genthon, M., Brugière, S., Triponney, P., Cobessi, D., Couté, Y., Jeannot, K., & Attrée, I. (2024). Pseudomonas aeruginosa MipA-MipB envelope proteins act as new sensors of polymyxins. MBio, 15(3), e0221123. https://doi.org/10.1128/mbio.02211-23
Anoud, M., Delagoutte, E., Helleu, Q., Brion, A., Duvernois-Berthet, E., As, M., Marques, X., Lamribet, K., Senamaud-Beaufort, C., Jourdren, L., Adrait, A., Heinrich, S., Toutirais, G., Hamlaoui, S., Gropplero, G., Giovannini, I., Ponger, L., Geze, M., Blugeon, C., … Concordet, J.-P. (2024). Comparative transcriptomics reveal a novel tardigrade-specific DNA-binding protein induced in response to ionizing radiation. ELife, 13, RP92621. https://doi.org/10.7554/eLife.92621
Bagdadi, N., Wu, J., Delaroche, J., Serre, L., Delphin, C., De Andrade, M., Carcel, M., Nawabi, H., Pinson, B., Vérin, C., Couté, Y., Gory-Fauré, S., Andrieux, A., Stoppin-Mellet, V., & Arnal, I. (2024). Stable GDP-tubulin islands rescue dynamic microtubules. The Journal of Cell Biology, 223(8), e202307074. https://doi.org/10.1083/jcb.202307074
Diaz-Barreiro, A., Cereghetti, G., Ortega Sánchez, F. G., Tonacini, J., Talabot-Ayer, D., Kieffer-Jaquinod, S., Kissling, V. M., Huard, A., Swale, C., Knowles, T. P. J., Couté, Y., Peter, M., Francés-Monerris, A., & Palmer, G. (2024). Oxidation-sensitive cysteines drive IL-38 amyloid formation. Cell Reports, 43(11), 114940. https://doi.org/10.1016/j.celrep.2024.114940
Torres-Romero, I., Légeret, B., Bertrand, M., Sorigue, D., Damm, A., Cuiné, S., Veillet, F., Blot, C., Brugière, S., Couté, Y., Garneau, M. G., Kotapati, H. K., Xin, Y., Xu, J., Bates, P. D., Thiam, A. R., Beisson, F., & Li-Beisson, Y. (2024). α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation. National Science Review, 11(12), nwae398. https://doi.org/10.1093/nsr/nwae398
Morin, C., Baudin-Baillieu, A., Van Long, F. N., Isaac, C., Bidou, L., Arbes, H., François, P., Pommier, R. M., Adrait, A., Saku, A., Gran-Ruaz, S., Machkouri, C., Vanbelle, C., Morichon, R., Boissan, M., Catez, F., Ferrari, A., Morel, A.-P., Couté, Y., … Marcel, V. (2024). Intricate ribosome composition and translational reprogramming in epithelial-mesenchymal transition. Proceedings of the National Academy of Sciences of the United States of America, 121(50), e2408114121. https://doi.org/10.1073/pnas.2408114121

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Svahn, J., Coudert, L., Streichenberger, N., Kraut, A., Gravier-Dumonceau-Mazelier, A., Rotard, L., Calemard-Michel, L., Menassa, R., Errazuriz-Cerda, E., Chalabreysse, L., Osseni, A., Vial, C., Jomir, L., Tronc, F., Le Duy, D., Bernard, E., Gache, V., Couté, Y., Jacquemond, V., … Leblanc, P. (2023). Immune-Mediated Rippling Muscle Disease Associated With Thymoma and Anti-MURC/Cavin-4 Autoantibodies. Neurology(R) Neuroimmunology & Neuroinflammation, 10(1), e200068. https://doi.org/10.1212/NXI.0000000000200068
da Silva Barreira, D., Laurent, J., Lourenço, J., Novion Ducassou, J., Couté, Y., Guzzo, J., & Rieu, A. (2023). Membrane vesicles released by Lacticaseibacillus casei BL23 inhibit the biofilm formation of Salmonella Enteritidis. Scientific Reports, 13(1), 1163. https://doi.org/10.1038/s41598-023-27959-9
Durand, S., Bruelle, M., Bourdelais, F., Bennychen, B., Blin-Gonthier, J., Isaac, C., Huyghe, A., Martel, S., Seyve, A., Vanbelle, C., Adrait, A., Couté, Y., Meyronet, D., Catez, F., Diaz, J.-J., Lavial, F., Ricci, E. P., Ducray, F., & Gabut, M. (2023). RSL24D1 sustains steady-state ribosome biogenesis and pluripotency translational programs in embryonic stem cells. Nature Communications, 14(1), 356. https://doi.org/10.1038/s41467-023-36037-7
Bodnar-Wachtel, M., Huber, A.-L., Gorry, J., Hacot, S., Burlet, D., Gérossier, L., Guey, B., Goutagny, N., Bartosch, B., Ballot, E., Lecuelle, J., Truntzer, C., Ghiringhelli, F., Py, B. F., Couté, Y., Ballesta, A., Lantuejoul, S., Hall, J., Tissier, A., & Petrilli, V. (2023). Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress. Life Science Alliance, 6(4), e202201494. https://doi.org/10.26508/lsa.202201494
Dushime, H., Moreno, S. G., Linard, C., Adrait, A., Couté, Y., Peltzer, J., Messiaen, S., Torres, C., Bensemmane, L., Lewandowski, D., Romeo, P.-H., Petit, V., & Gault, N. (2023). Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration. Stem Cell Research & Therapy, 14(1), 201. https://doi.org/10.1186/s13287-023-03425-1
Shamseddine, L., Roblin, C., Veyrier, I., Basset, C., De Macedo, L., Boyeldieu, A., Maresca, M., Nicoletti, C., Brasseur, G., Kieffer-Jaquinod, S., Courvoisier-Dezord, É., Amouric, A., Carpentier, P., Campo, N., Bergé, M., Polard, P., Perrier, J., Duarte, V., & Lafond, M. (2023). Mechanistic and functional aspects of the Ruminococcin C sactipeptide isoforms. IScience, 26(9), 107563. https://doi.org/10.1016/j.isci.2023.107563
Marziali, F., Song, Y., Nguyen, X.-N., Belmudes, L., Burlaud-Gaillard, J., Roingeard, P., Couté, Y., & Cimarelli, A. (2023). A Proteomics-Based Approach Identifies the NEDD4 Adaptor NDFIP2 as an Important Regulator of Ifitm3 Levels. Viruses, 15(10), 1993. https://doi.org/10.3390/v15101993
Kairouani, A., Pontier, D., Picart, C., Mounet, F., Martinez, Y., Le-Bot, L., Fanuel, M., Hammann, P., Belmudes, L., Merret, R., Azevedo, J., Carpentier, M.-C., Gagliardi, D., Couté, Y., Sibout, R., Bies-Etheve, N., & Lagrange, T. (2023). Cell-type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis. ELife, 12, RP88207. https://doi.org/10.7554/eLife.88207
Robert, V. J., Caron, M., Gely, L., Adrait, A., Pakulska, V., Couté, Y., Chevalier, M., Riedel, C. G., Bedet, C., & Palladino, F. (2023). SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans. Development (Cambridge, England), 150(21), dev201755. https://doi.org/10.1242/dev.201755
Zafirov, D., Giovinazzo, N., Lecampion, C., Field, B., Ducassou, J. N., Couté, Y., Browning, K. S., Robaglia, C., & Gallois, J.-L. (2023). Arabidopsis eIF4E1 protects the translational machinery during TuMV infection and restricts virus accumulation. PLoS Pathogens, 19(11), e1011417. https://doi.org/10.1371/journal.ppat.1011417
Huna, A., Flaman, J.-M., Lodillinsky, C., Zhu, K., Makulyte, G., Pakulska, V., Coute, Y., Ruisseaux, C., Saintigny, P., Hernandez-Vargas, H., Defossez, P.-A., Boissan, M., Martin, N., & Bernard, D. (2023). RSK3 switches cell fate: from stress-induced senescence to malignant progression. Journal of Experimental & Clinical Cancer Research: CR, 42(1), 318. https://doi.org/10.1186/s13046-023-02909-5

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Capizzi, M., Carpentier, R., Denarier, E., Adrait, A., Kassem, R., Mapelli, M., Couté, Y., & Humbert, S. (2022). Developmental defects in Huntington’s disease show that axonal growth and microtubule reorganization require NUMA1. Neuron, 110(1), 36-50.e5. https://doi.org/10.1016/j.neuron.2021.10.033
Caron, M., Gely, L., Garvis, S., Adrait, A., Couté, Y., Palladino, F., & Fabrizio, P. (2022). Loss of SET1/COMPASS methyltransferase activity reduces lifespan and fertility in Caenorhabditis elegans. Life Science Alliance, 5(3), e202101140. https://doi.org/10.26508/lsa.202101140
Gfrerer, S., Winkler, D., Novion Ducassou, J., Couté, Y., Rachel, R., & Gescher, J. (2022). A Micrarchaeon Isolate Is Covered by a Proteinaceous S-Layer. Applied and Environmental Microbiology, 88(5), e01553-21. https://doi.org/10.1128/aem.01553-21
Ben Amar, D., Thoinet, K., Villalard, B., Imbaud, O., Costechareyre, C., Jarrosson, L., Reynaud, F., Novion Ducassou, J., Couté, Y., Brunet, J.-F., Combaret, V., Corradini, N., Delloye-Bourgeois, C., & Castellani, V. (2022). Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model. Nature Communications, 13(1), 2549. https://doi.org/10.1038/s41467-022-30237-3
Vigetti, L., Labouré, T., Roumégous, C., Cannella, D., Touquet, B., Mayer, C., Couté, Y., Frénal, K., Tardieux, I., & Renesto, P. (2022). The BCC7 Protein Contributes to the Toxoplasma Basal Pole by Interfacing between the MyoC Motor and the IMC Membrane Network. International Journal of Molecular Sciences, 23(11), 5995. https://doi.org/10.3390/ijms23115995
Dragic, H., Barthelaix, A., Duret, C., Le Goupil, S., Laprade, H., Martin, S., Brugière, S., Couté, Y., Machon, C., Guitton, J., Rudewicz, J., Hofman, P., Lebecque, S., Chaveroux, C., Ferraro-Peyret, C., Renno, T., & Manié, S. N. (2022). The hexosamine pathway and coat complex II promote malignant adaptation to nutrient scarcity. Life Science Alliance, 5(7), e202101334. https://doi.org/10.26508/lsa.202101334
Job, V., Gomez-Valero, L., Renier, A., Rusniok, C., Bouillot, S., Chenal-Francisque, V., Gueguen, E., Adrait, A., Robert-Genthon, M., Jeannot, K., Panchev, P., Elsen, S., Fauvarque, M.-O., Couté, Y., Buchrieser, C., & Attrée, I. (2022). Genomic erosion and horizontal gene transfer shape functional differences of the ExlA toxin in Pseudomonas spp. IScience, 25(7), 104596. https://doi.org/10.1016/j.isci.2022.104596
Mladenov, P., Zasheva, D., Planchon, S., Leclercq, C. C., Falconet, D., Moyet, L., Brugière, S., Moyankova, D., Tchorbadjieva, M., Ferro, M., Rolland, N., Renaut, J., Djilianov, D., & Deng, X. (2022). Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea rhodopensis. International Journal of Molecular Sciences, 23(15), 8520. https://doi.org/10.3390/ijms23158520
Nyonda, M. A., Boyer, J.-B., Belmudes, L., Krishnan, A., Pino, P., Couté, Y., Brochet, M., Meinnel, T., Soldati-Favre, D., & Giglione, C. (2022). N-acetylation of secreted proteins in Apicomplexa is widespread and is independent of the ER acetyl-CoA transporter AT1. Journal of Cell Science, 135(15), jcs259811. https://doi.org/10.1242/jcs.259811
Ruedas, R., Muthukumar, S. S., Kieffer-Jaquinod, S., Gillet, F.-X., Fenel, D., Effantin, G., Pfannschmidt, T., Couté, Y., Blanvillain, R., & Cobessi, D. (2022). Three-Dimensional Envelope and Subunit Interactions of the Plastid-Encoded RNA Polymerase from Sinapis alba. International Journal of Molecular Sciences, 23(17), 9922. https://doi.org/10.3390/ijms23179922
Lukinović, V., Hausmann, S., Roth, G. S., Oyeniran, C., Ahmad, T., Tsao, N., Brickner, J. R., Casanova, A. G., Chuffart, F., Benitez, A. M., Vayr, J., Rodell, R., Tardif, M., Jansen, P. W. T. C., Couté, Y., Vermeulen, M., Hainaut, P., Mazur, P. K., Mosammaparast, N., & Reynoird, N. (2022). SMYD3 Impedes Small Cell Lung Cancer Sensitivity to Alkylation Damage through RNF113A Methylation-Phosphorylation Cross-talk. Cancer Discovery, 12(9), 2158–2179. https://doi.org/10.1158/2159-8290.CD-21-0205
Vilallongue, N., Schaeffer, J., Hesse, A.-M., Delpech, C., Blot, B., Paccard, A., Plissonnier, E., Excoffier, B., Couté, Y., Belin, S., & Nawabi, H. (2022). Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nature Communications, 13(1), 6040. https://doi.org/10.1038/s41467-022-33799-4
Lacroux, J., Atteia, A., Brugière, S., Couté, Y., Vallon, O., Steyer, J.-P., & van Lis, R. (2022). Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp. Frontiers in Microbiology, 13, 1029828. https://doi.org/10.3389/fmicb.2022.1029828
da Silva Barreira, D., Lapaquette, P., Novion Ducassou, J., Couté, Y., Guzzo, J., & Rieu, A. (2022). Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23. MBio, 13(5), e0237522. https://doi.org/10.1128/mbio.02375-22
Lupette, J., Tardif, M., Brugière, S., Couté, Y., Salvaing, J., & Maréchal, E. (2022). Quantitative proteomic analyses reveal the impact of nitrogen starvation on the proteome of the model diatom Phaeodactylum tricornutum. Proteomics, 22(22), e2200155. https://doi.org/10.1002/pmic.202200155
Barnault, R., Verzeroli, C., Fournier, C., Michelet, M., Redavid, A. R., Chicherova, I., Plissonnier, M.-L., Adrait, A., Khomich, O., Chapus, F., Richaud, M., Hervieu, M., Reiterer, V., Centonze, F. G., Lucifora, J., Bartosch, B., Rivoire, M., Farhan, H., Couté, Y., … Parent, R. (2022). Hepatic inflammation elicits production of proinflammatory netrin-1 through exclusive activation of translation. Hepatology (Baltimore, Md.), 76(5), 1345–1359. https://doi.org/10.1002/hep.32446
Leisico, F., Omeiri, J., Le Narvor, C., Beaudouin, J., Hons, M., Fenel, D., Schoehn, G., Couté, Y., Bonnaffé, D., Sadir, R., Lortat-Jacob, H., & Wild, R. (2022). Structure of the human heparan sulfate polymerase complex EXT1-EXT2. Nature Communications, 13(1), 7110. https://doi.org/10.1038/s41467-022-34882-6
Ielasi, F. S., Ternifi, S., Fontaine, E., Iuso, D., Couté, Y., & Palencia, A. (2022). Human histone pre-mRNA assembles histone or canonical mRNA-processing complexes by overlapping 3’-end sequence elements. Nucleic Acids Research, 50(21), 12425–12443. https://doi.org/10.1093/nar/gkac878
Binda, O., Juillard, F., Ducassou, J. N., Kleijwegt, C., Paris, G., Didillon, A., Baklouti, F., Corpet, A., Couté, Y., Côté, J., & Lomonte, P. (2022). SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Science Alliance, 6(1), e202201429. https://doi.org/10.26508/lsa.202201429

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Takata-Tsuji, F., Chounlamountri, N., Do, L.-D., Philippot, C., Novion Ducassou, J., Couté, Y., Ben Achour, S., Honnorat, J., Place, C., & Pascual, O. (2021). Microglia modulate gliotransmission through the regulation of VAMP2 proteins in astrocytes. Glia, 69(1), 61–72. https://doi.org/10.1002/glia.23884
Kerjouan, A., Boyault, C., Oddou, C., Hiriart-Bryant, E., Grichine, A., Kraut, A., Pezet, M., Balland, M., Faurobert, E., Bonnet, I., Coute, Y., Fourcade, B., Albiges-Rizo, C., & Destaing, O. (2021). Control of SRC molecular dynamics encodes distinct cytoskeletal responses by specifying signaling pathway usage. Journal of Cell Science, 134(2), jcs254599. https://doi.org/10.1242/jcs.254599
Vellino, S., Oddou, C., Rivier, P., Boyault, C., Hiriart-Bryant, E., Kraut, A., Martin, R., Coute, Y., Knölker, H.-J., Valverde, M. A., Albigès-Rizo, C., & Destaing, O. (2021). Cross-talk between the calcium channel TRPV4 and reactive oxygen species interlocks adhesive and degradative functions of invadosomes. Journal of Cell Biology, 220(2), e201910079. https://doi.org/10.1083/jcb.201910079
van Lis, R., Couté, Y., Brugière, S., Tourasse, N. J., Laurent, B., Nitschke, W., Vallon, O., & Atteia, A. (2021). Phylogenetic and functional diversity of aldehyde-alcohol dehydrogenases in microalgae. Plant Molecular Biology, 105(4–5), 497–511. https://doi.org/10.1007/s11103-020-01105-9
Chiari, L., Carpentier, P., Kieffer-Jaquinod, S., Gogny, A., Perard, J., Ravanel, S., Cobessi, D., Ménage, S., Dumas, R., & Hamelin, O. (2021). LEAFY protein crystals with a honeycomb structure as a platform for selective preparation of outstanding stable bio-hybrid materials. Nanoscale, 13(19), 8901–8908. https://doi.org/10.1039/D1NR00268F
Montillet, J.-L., Rondet, D., Brugière, S., Henri, P., Rumeau, D., Reichheld, J.-P., Couté, Y., Leonhardt, N., & Rey, P. (2021). Plastidial and cytosolic thiol reductases participate in the control of stomatal functioning. Plant, Cell & Environment, 44(5), 1417–1435. https://doi.org/10.1111/pce.14013
Härrer, D., Windhorst, C., Böhner, N., Novion Ducassou, J., Couté, Y., & Gescher, J. (2021). Production of acetoin from renewable resources under heterotrophic and mixotrophic conditions. Bioresource Technology, 329, 124866. https://doi.org/10.1016/j.biortech.2021.124866
Pallavicini, G., Gai, M., Iegiani, G., Berto, G. E., Adrait, A., Couté, Y., & Di Cunto, F. (2021). Goldberg–Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis. Journal of Cell Science, 134(11), jcs250902. https://doi.org/10.1242/jcs.250902
Edel, M., Sturm, G., Sturm-Richter, K., Wagner, M., Ducassou, J. N., Couté, Y., Horn, H., & Gescher, J. (2021). Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis. Biotechnology for Biofuels, 14(1), 130. https://doi.org/10.1186/s13068-021-01981-3
Humbert, P., Brennan, M. Á., De Lima, J., Brion, R., Adrait, A., Charrier, C., Brulin, B., Trichet, V., Couté, Y., Blanchard, F., & Layrolle, P. (2021). Apoptotic mesenchymal stromal cells support osteoclastogenesis while inhibiting multinucleated giant cells formation in vitro. Scientific Reports, 11(1), 12144. https://doi.org/10.1038/s41598-021-91258-4
Pipier, A., Devaux, A., Lavergne, T., Adrait, A., Couté, Y., Britton, S., Calsou, P., Riou, J. F., Defrancq, E., & Gomez, D. (2021). Constrained G4 structures unveil topology specificity of known and new G4 binding proteins. Scientific Reports, 11(1), 13469. https://doi.org/10.1038/s41598-021-92806-8
Do, L.-D., Moritz, C. P., Muñiz-Castrillo, S., Pinto, A.-L., Tholance, Y., Brugiere, S., Couté, Y., Stoevesandt, O., Taussig, M. J., Rogemond, V., Vogrig, A., Joubert, B., Ferraud, K., Camdessanché, J.-P., Antoine, J.-C., & Honnorat, J. (2021). Argonaute Autoantibodies as Biomarkers in Autoimmune Neurologic Diseases. Neurology - Neuroimmunology Neuroinflammation, 8(5), e1032. https://doi.org/10.1212/NXI.0000000000001032
Schmid, F., Novion Ducassou, J., Couté, Y., & Gescher, J. (2021). Developing Rhodobacter sphaeroides for cathodic biopolymer production. Bioresource Technology, 336, 125340. https://doi.org/10.1016/j.biortech.2021.125340
Jalabert, A., Reininger, L., Berger, E., Coute, Y., Meugnier, E., Forterre, A., Errazuriz-Cerda, E., Geloen, A., Aouadi, M., Bouzakri, K., Rieusset, J., & Rome, S. (2021). Profiling of ob/ob mice skeletal muscle exosome-like vesicles demonstrates combined action of miRNAs, proteins and lipids to modulate lipid homeostasis in recipient cells. Scientific Reports, 11(1), 21626. https://doi.org/10.1038/s41598-021-00983-3
Häge, S., Büscher, N., Pakulska, V., Hahn, F., Adrait, A., Krauter, S., Borst, E. M., Schlötzer-Schrehardt, U., Couté, Y., Plachter, B., & Marschall, M. (2021). The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus. Cells, 10(11), 3119. https://doi.org/10.3390/cells10113119
Bouroumeau, A., Bussot, L., Hamaidia, S., Garcìa-Sandoval, A., Bergan-Dahl, A., Betton-Fraisse, P., Duley, S., Fournier, C., Aucagne, R., Adrait, A., Couté, Y., McLeer, A., Col, E., David-Boudet, L., Raskovalova, T., Jacob, M.-C., Vettier, C., Chevalier, S., Carras, S., … Emadali, A. (2021). CYCLON and NPM1 Cooperate within an Oncogenic Network Predictive of R-CHOP Response in DLBCL. Cancers, 13(23), 5900. https://doi.org/10.3390/cancers13235900
Boulan, B., Ravanello, C., Peyrel, A., Bosc, C., Delphin, C., Appaix, F., Denarier, E., Kraut, A., Jaquier-Sarlin, M., Fournier, A., Andrieux, A., Gory-Fauré, S., & Deloulme, J.-C. (2021). CRMP4-mediated fornix development involves semaphorin-3E signaling pathway. ELife, 10, e70361. https://doi.org/10.7554/eLife.70361

2020

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Lezzerini, M., Penzo, M., O’Donohue, M.-F., Marques Dos Santos Vieira, C., Saby, M., Elfrink, H. L., Diets, I. J., Hesse, A.-M., Couté, Y., Gastou, M., Nin-Velez, A., Nikkels, P. G. J., Olson, A. N., Zonneveld-Huijssoon, E., Jongmans, M. C. J., Zhang, G., van Weeghel, M., Houtkooper, R. H., Wlodarski, M. W., … MacInnes, A. W. (2020). Ribosomal protein gene RPL9 variants can differentially impair ribosome function and cellular metabolism. Nucleic Acids Research, 48(2), 770–787. https://doi.org/10.1093/nar/gkz1042
van Lis, R., Brugière, S., Baffert, C., Couté, Y., Nitschke, W., & Atteia, A. (2020). Hybrid cluster proteins in a photosynthetic microalga. The FEBS Journal, 287(4), 721–735. https://doi.org/10.1111/febs.15025
Began, J., Cordier, B., Březinová, J., Delisle, J., Hexnerová, R., Srb, P., Rampírová, P., Kožíšek, M., Baudet, M., Couté, Y., Galinier, A., Veverka, V., Doan, T., & Strisovsky, K. (2020). Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. The EMBO Journal, 39(10). https://doi.org/10.15252/embj.2019102935
Hahn, F., Niesar, A., Wangen, C., Wild, M., Grau, B., Herrmann, L., Capci, A., Adrait, A., Couté, Y., Tsogoeva, S. B., & Marschall, M. (2020). Target verification of artesunate-related antiviral drugs: Assessing the role of mitochondrial and regulatory proteins by click chemistry and fluorescence labeling. Antiviral Research, 180, 104861. https://doi.org/10.1016/j.antiviral.2020.104861
Roblin, C., Chiumento, S., Bornet, O., Nouailler, M., Müller, C. S., Jeannot, K., Basset, C., Kieffer-Jaquinod, S., Couté, Y., Torelli, S., Le Pape, L., Schünemann, V., Olleik, H., De La Villeon, B., Sockeel, P., Di Pasquale, E., Nicoletti, C., Vidal, N., Poljak, L., … Duarte, V. (2020). The unusual structure of Ruminococcin C1 antimicrobial peptide confers clinical properties. Proceedings of the National Academy of Sciences, 117(32), 19168–19177. https://doi.org/10.1073/pnas.2004045117
Nguyen, M. V. C., Courtier, A., Adrait, A., Defendi, F., Couté, Y., Baillet, A., Guigue, L., Gottenberg, J.-E., Dumestre-Pérard, C., Brun, V., & Gaudin, P. (2020). Fetuin-A and thyroxin binding globulin predict rituximab response in rheumatoid arthritis patients with insufficient response to anti-TNFα. Clinical Rheumatology, 39(9), 2553–2562. https://doi.org/10.1007/s10067-020-05030-6
Sentausa, E., Basso, P., Berry, A., Adrait, A., Bellement, G., Couté, Y., Lory, S., Elsen, S., & Attrée, I. (2020). Insertion sequences drive the emergence of a highly adapted human pathogen. Microbial Genomics, 6(9). https://doi.org/10.1099/mgen.0.000265
Di Giovanni, D., Lepetit, D., Guinet, B., Bennetot, B., Boulesteix, M., Couté, Y., Bouchez, O., Ravallec, M., & Varaldi, J. (2020). A Behavior-Manipulating Virus Relative as a Source of Adaptive Genes for Drosophila Parasitoids. Molecular Biology and Evolution, 37(10), 2791–2807. https://doi.org/10.1093/molbev/msaa030
Chabrolles, H., Auclair, H., Vegna, S., Lahlali, T., Pons, C., Michelet, M., Couté, Y., Belmudes, L., Chadeuf, G., Kim, Y., Di Bernardo, A., Jalaguier, P., Cosset, F.-L., Fusil, F., Rivoire, M., Arnold, L. D., Lopatin, U., Combet, C., Zoulim, F., … Salvetti, A. (2020). Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production. PLoS Pathogens, 16(11), e1008593. https://doi.org/10.1371/journal.ppat.1008593
Dia, M., Gomez, L., Thibault, H., Tessier, N., Leon, C., Chouabe, C., Ducreux, S., Gallo-Bona, N., Tubbs, E., Bendridi, N., Chanon, S., Leray, A., Belmudes, L., Couté, Y., Kurdi, M., Ovize, M., Rieusset, J., & Paillard, M. (2020). Reduced reticulum–mitochondria Ca2+ transfer is an early and reversible trigger of mitochondrial dysfunctions in diabetic cardiomyopathy. Basic Research in Cardiology, 115(6), 74. https://doi.org/10.1007/s00395-020-00835-7
Ngo, T.-D., Perdu, C., Jneid, B., Ragno, M., Novion Ducassou, J., Kraut, A., Couté, Y., Stopford, C., Attrée, I., Rietsch, A., & Faudry, E. (2020). The PopN Gate-keeper Complex Acts on the ATPase PscN to Regulate the T3SS Secretion Switch from Early to Middle Substrates in Pseudomonas aeruginosa. Journal of Molecular Biology, 432(24), 166690. https://doi.org/10.1016/j.jmb.2020.10.024
Del Corpo, D., Fullone, M. R., Miele, R., Lafond, M., Pontiggia, D., Grisel, S., Kieffer‐Jaquinod, S., Giardina, T., Bellincampi, D., & Lionetti, V. (2020). AtPME17 is a functional Arabidopsis thaliana pectin methylesterase regulated by its PRO region that triggers PME activity in the resistance to Botrytis cinerea. Molecular Plant Pathology, 21(12), 1620–1633. https://doi.org/10.1111/mpp.13002

2019

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Siebert, C., Lindgren, H., Ferré, S., Villers, C., Boisset, S., Perard, J., Sjöstedt, A., Maurin, M., Brochier-Armanet, C., Couté, Y., & Renesto, P. (2019). Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation. Emerging Microbes & Infections, 8(1), 808–822. https://doi.org/10.1080/22221751.2019.1615848
Pinaud, S., Portet, A., Allienne, J.-F., Belmudes, L., Saint-Beat, C., Arancibia, N., Galinier, R., Du Pasquier, L., Duval, D., & Gourbal, B. (2019). Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata. Developmental and Comparative Immunology, 92, 238–252. https://doi.org/10.1016/j.dci.2018.12.001
Steingruber, M., Keller, L., Socher, E., Ferre, S., Hesse, A.-M., Couté, Y., Hahn, F., Büscher, N., Plachter, B., Sticht, H., & Marschall, M. (2019). Cyclins B1, T1, and H differ in their molecular mode of interaction with cytomegalovirus protein kinase pUL97. The Journal of Biological Chemistry, 294(15), 6188–6203. https://doi.org/10.1074/jbc.RA118.007049
Wei, J., Kishton, R. J., Angel, M., Conn, C. S., Dalla-Venezia, N., Marcel, V., Vincent, A., Catez, F., Ferré, S., Ayadi, L., Marchand, V., Dersh, D., Gibbs, J. S., Ivanov, I. P., Fridlyand, N., Couté, Y., Diaz, J.-J., Qian, S.-B., Staudt, L. M., … Yewdell, J. W. (2019). Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance. Molecular Cell, 73(6), 1162-1173.e5. https://doi.org/10.1016/j.molcel.2018.12.020
Lupette, J., Jaussaud, A., Seddiki, K., Morabito, C., Brugière, S., Schaller, H., Kuntz, M., Putaux, J.-L., Jouneau, P.-H., Rébeillé, F., Falconet, D., Couté, Y., Jouhet, J., Tardif, M., Salvaing, J., & Maréchal, E. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research, 38, 101415. https://doi.org/10.1016/j.algal.2019.101415
Do, L. D., Gupton, S. L., Tanji, K., Bastien, J., Brugière, S., Couté, Y., Quadrio, I., Rogemond, V., Fabien, N., Desestret, V., & Honnorat, J. (2019). TRIM9 and TRIM67 Are New Targets in Paraneoplastic Cerebellar Degeneration. Cerebellum (London, England), 18(2), 245–254. https://doi.org/10.1007/s12311-018-0987-5
Hajj Chehade, M., Pelosi, L., Fyfe, C. D., Loiseau, L., Rascalou, B., Brugière, S., Kazemzadeh, K., Vo, C.-D.-T., Ciccone, L., Aussel, L., Couté, Y., Fontecave, M., Barras, F., Lombard, M., & Pierrel, F. (2019). A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone. Cell Chemical Biology, 26(4), 482-492.e7. https://doi.org/10.1016/j.chembiol.2018.12.001
Sonego, M., Pellarin, I., Costa, A., Vinciguerra, G. L. R., Coan, M., Kraut, A., D’Andrea, S., Dall’Acqua, A., Castillo-Tong, D. C., Califano, D., Losito, S., Spizzo, R., Couté, Y., Vecchione, A., Belletti, B., Schiappacassi, M., & Baldassarre, G. (2019). USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Science Advances, 5(5), eaav3235. https://doi.org/10.1126/sciadv.aav3235
Bhalla, P., Vernekar, D. V., Gilquin, B., Couté, Y., & Bhargava, P. (2019). Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II. Gene, 702, 205–214. https://doi.org/10.1016/j.gene.2018.12.037
Darrieutort-Laffite, C., Arnolfo, P., Garraud, T., Adrait, A., Couté, Y., Louarn, G., Trichet, V., Layrolle, P., Le Goff, B., & Blanchard, F. (2019). Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. Journal of Clinical Medicine, 8(10), 1544. https://doi.org/10.3390/jcm8101544
Desroches-Castan, A., Tillet, E., Ricard, N., Ouarné, M., Mallet, C., Belmudes, L., Couté, Y., Boillot, O., Scoazec, J.-Y., Bailly, S., & Feige, J.-J. (2019). Bone Morphogenetic Protein 9 Is a Paracrine Factor Controlling Liver Sinusoidal Endothelial Cell Fenestration and Protecting Against Hepatic Fibrosis. Hepatology (Baltimore, Md.), 70(4), 1392–1408. https://doi.org/10.1002/hep.30655
Chapelle, J., Sorokina, O., McLean, C., Salemme, V., Alfieri, A., Angelini, C., Morellato, A., Adrait, A., Menna, E., Matteoli, M., Couté, Y., Ala, U., Turco, E., Defilippi, P., & Armstrong, J. D. (2019). Dissecting the Shared and Context-Dependent Pathways Mediated by the p140Cap Adaptor Protein in Cancer and in Neurons. Frontiers in Cell and Developmental Biology, 7, 222. https://doi.org/10.3389/fcell.2019.00222
Moulin, M., Mossou, E., Signor, L., Kieffer-Jaquinod, S., Kwaambwa, H. M., Nermark, F., Gutfreund, P., Mitchell, E. P., Haertlein, M., Forsyth, V. T., & Rennie, A. R. (2019). Towards a molecular understanding of the water purification properties of Moringa seed proteins. Journal of Colloid and Interface Science, 554, 296–304. https://doi.org/10.1016/j.jcis.2019.06.071
Dell’Aglio, E., Giustini, C., Kraut, A., Couté, Y., Costa, A., Decros, G., Gibon, Y., Mazars, C., Matringe, M., Finazzi, G., & Curien, G. (2019). Identification of the Arabidopsis Calmodulin-Dependent NAD + Kinase That Sustains the Elicitor-Induced Oxidative Burst. Plant Physiology, 181(4), 1449–1458. https://doi.org/10.1104/pp.19.00912
Beurton, F., Stempor, P., Caron, M., Appert, A., Dong, Y., Chen, R. A., Cluet, D., Couté, Y., Herbette, M., Huang, N., Polveche, H., Spichty, M., Bedet, C., Ahringer, J., & Palladino, F. (2019). Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans. Nucleic Acids Research, 47(21), 11164–11180. https://doi.org/10.1093/nar/gkz880