EDyP
238648
TK2D2GKR
2025
1
apa
50
date
asc
1494
https://www.edyp.fr/web/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-f1afbb04b4d3ca88400e799098bc5292%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ATA9BBNG%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Geshkovski%20et%20al.%22%2C%22parsedDate%22%3A%222025%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGeshkovski%2C%20V.%2C%20Hijazi%2C%20H.%2C%20Manessier%2C%20J.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Cour%26%23xE7%3Bon%2C%20M.%2C%20Vachon%2C%20G.%2C%20Pflieger%2C%20D.%2C%20%26amp%3B%20Carles%2C%20C.%20C.%20%282025%29.%20Quantitative%20Profiling%20of%20Histone%20Variants%20and%20Posttranslational%20Modifications%20by%20Tandem%20Mass%20Spectrometry%20in%20Arabidopsis.%20%3Ci%3EMethods%20in%20Molecular%20Biology%20%28Clifton%2C%20N.J.%29%3C%5C%2Fi%3E%2C%20%3Ci%3E2873%3C%5C%2Fi%3E%2C%2019%26%23x2013%3B38.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2F978-1-0716-4228-3_2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2F978-1-0716-4228-3_2%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Quantitative%20Profiling%20of%20Histone%20Variants%20and%20Posttranslational%20Modifications%20by%20Tandem%20Mass%20Spectrometry%20in%20Arabidopsis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vangeli%22%2C%22lastName%22%3A%22Geshkovski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hassan%22%2C%22lastName%22%3A%22Hijazi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julie%22%2C%22lastName%22%3A%22Manessier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Cour%5Cu00e7on%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Vachon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Pflieger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristel%20C.%22%2C%22lastName%22%3A%22Carles%22%7D%5D%2C%22abstractNote%22%3A%22Histone%20dynamics%20constitute%20an%20important%20layer%20of%20gene%20regulations%20associated%20with%20development%20and%20growth%20in%20multicellular%20eukaryotes.%20They%20also%20stand%20as%20key%20determinants%20of%20plant%20responses%20to%20environmental%20changes.%20Histone%20dynamics%20include%20the%20exchange%20of%20histone%20variants%20as%20well%20as%20post-translational%20modifications%20of%20their%20amino%20acid%20residues%20%28such%20as%20acetylation%20and%20mono%5C%2Fdi%5C%2Ftrimethylation%29%2C%20commonly%20referred%20to%20as%20histone%20marks.%20Investigating%20histone%20dynamics%20with%20a%20focus%20on%20combinatorial%20changes%20occurring%20at%20their%20residues%20will%20greatly%20help%20unravel%20how%20plants%20achieve%20phenotypic%20plasticity.Mass%20spectrometry%20%28MS%29%20analysis%20offers%20unequaled%20resolution%20of%20the%20abundance%20of%20histone%20variants%20and%20of%20their%20marks.%20Indeed%2C%20relative%20to%20other%20techniques%20such%20as%20western%20blot%20or%20genome-wide%20profiling%2C%20this%20powerful%20technique%20allows%20quantifying%20the%20relative%20abundances%20of%20histone%20forms%2C%20as%20well%20as%20revealing%20coexisting%20marks%20on%20the%20same%20histone%20molecule.%20Yet%2C%20while%20MS-based%20histone%20analysis%20has%20proven%20efficient%20in%20several%20animals%20and%20other%20model%20organisms%2C%20this%20method%20stands%20out%20as%20more%20challenging%20in%20plants.%20One%20major%20challenge%20is%20the%20isolation%20of%20sufficient%20amounts%20of%20pure%2C%20high-quality%20histones%2C%20likely%20rendered%20difficult%20by%20the%20presence%20of%20the%20cell%20wall%2C%20for%20sufficiently%20deep%20and%20resolutive%20identification%20of%20histone%20species.In%20this%20chapter%2C%20we%20describe%20a%20straightforward%20MS-based%20proteomic%20method%2C%20implemented%20to%20characterize%20histone%20marks%20from%20Arabidopsis%20thaliana%20seedling%20tissues%20and%20cell%20culture%20suspensions.%20After%20acid%20extraction%20of%20histones%2C%20in%20vitro%20propionylation%20of%20free%20lysine%20residues%2C%20and%20digestion%20with%20trypsin%2C%20a%20treatment%20at%20highly%20basic%20pH%20allows%20obtaining%20sharp%20spectral%20signals%20of%20biologically%20relevant%20histone%20peptide%20forms.The%20method%20workflow%20described%20here%20shall%20be%20used%20to%20measure%20changes%20in%20histone%20marks%20between%20Arabidopsis%20thaliana%20genotypes%2C%20along%20developmental%20time-courses%2C%20or%20upon%20various%20stresses%20and%20treatments.%22%2C%22date%22%3A%222025%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1007%5C%2F978-1-0716-4228-3_2%22%2C%22ISSN%22%3A%221940-6029%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%227KBU675S%22%5D%2C%22dateModified%22%3A%222024-11-25T07%3A41%3A48Z%22%7D%7D%2C%7B%22key%22%3A%22STS75ZDG%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Santini%20et%20al.%22%2C%22parsedDate%22%3A%222025-01-10%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESantini%2C%20S.%2C%20Lartigue%2C%20A.%2C%20Alempic%2C%20J.-M.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Belmudes%2C%20L.%2C%20Brazelton%2C%20W.%20J.%2C%20Lang%2C%20S.%20Q.%2C%20Claverie%2C%20J.-M.%2C%20Legendre%2C%20M.%2C%20%26amp%3B%20Abergel%2C%20C.%20%282025%29.%20Pacmanvirus%20isolated%20from%20the%20Lost%20City%20hydrothermal%20field%20extends%20the%20concept%20of%20transpoviron%20beyond%20the%20family%20Mimiviridae.%20%3Ci%3EThe%20ISME%20Journal%3C%5C%2Fi%3E%2C%20wraf002.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fismejo%5C%2Fwraf002%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fismejo%5C%2Fwraf002%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pacmanvirus%20isolated%20from%20the%20Lost%20City%20hydrothermal%20field%20extends%20the%20concept%20of%20transpoviron%20beyond%20the%20family%20Mimiviridae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S%5Cu00e9bastien%22%2C%22lastName%22%3A%22Santini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Audrey%22%2C%22lastName%22%3A%22Lartigue%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Marie%22%2C%22lastName%22%3A%22Alempic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%20J.%22%2C%22lastName%22%3A%22Brazelton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Susan%20Q.%22%2C%22lastName%22%3A%22Lang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Michel%22%2C%22lastName%22%3A%22Claverie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthieu%22%2C%22lastName%22%3A%22Legendre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chantal%22%2C%22lastName%22%3A%22Abergel%22%7D%5D%2C%22abstractNote%22%3A%22The%20microbial%20sampling%20of%20submarine%20hydrothermal%20vents%20remains%20challenging%2C%20with%20even%20fewer%20studies%20focused%20on%20viruses.%20Here%20we%20report%20the%20first%20isolation%20of%20a%20eukaryotic%20virus%20from%20the%20Lost%20City%20hydrothermal%20field%2C%20by%20co-culture%20with%20the%20laboratory%20host%20Acanthamoeba%20castellanii.%20This%20virus%2C%20named%20pacmanvirus%20lostcity%2C%20is%20closely%20related%20to%20previously%20isolated%20pacmanviruses%20%28strains%20A23%20and%20S19%29%2C%20clustering%20in%20a%20divergent%20clade%20within%20the%20long-established%20family%20Asfarviridae.%20Its%20icosahedral%20particles%20are%20200%5Cu00a0nm%20in%20diameter%2C%20with%20an%20electron-dense%20core%20surrounded%20by%20an%20inner%20membrane.%20Its%20genome%20of%20395%5Cu2009708%5Cu00a0bp%20%2833%25%20G%5Cu2009%2B%5Cu2009C%29%20is%20predicted%20to%20encode%20473%20proteins.%20However%2C%20besides%20these%20standard%20properties%2C%20pacmanvirus%20lostcity%20was%20found%20associated%20with%20a%20new%20type%20of%20selfish%20genetic%20element%2C%207%5Cu00a0kb%20in%20length%2C%20whose%20architecture%20and%20gene%20content%20are%20reminiscent%20of%20those%20of%20transpovirons%2C%20hitherto%20specific%20to%20the%20family%20Mimiviridae.%20Like%20previously%20described%20transpovirons%2C%20this%20element%20propagates%20as%20an%20episome%20within%20its%20host%20virus%20particles%20and%20exhibits%20partial%20recombination%20with%20its%20genome.%20In%20addition%2C%20an%20unrelated%202%5Cu00a0kb%20long%20episome%20was%20also%20associated%20with%20pacmanvirus%20lostcity.%20Together%2C%20the%20transpoviron%20and%20the%202%5Cu00a0kb%20episome%20might%20participate%20to%20exchanges%20between%20pacmanviruses%20and%20other%20large%20DNA%20virus%20families.%20It%20remains%20to%20be%20elucidated%20if%20the%20presence%20of%20these%20mobile%20genetic%20elements%20is%20restricted%20to%20pacmanviruses%20or%20was%20simply%20overlooked%20in%20other%20members%20of%20the%20Asfarviridae.%22%2C%22date%22%3A%222025-01-10%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fismejo%5C%2Fwraf002%22%2C%22ISSN%22%3A%221751-7370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-01-13T07%3A59%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22H3VNLXNB%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Dannay%20et%20al.%22%2C%22parsedDate%22%3A%222025-02-18%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDannay%2C%20M.%2C%20Bertin%2C%20C.%2C%20Cavallari%2C%20E.%2C%20Albanese%2C%20P.%2C%20Tolleter%2C%20D.%2C%20Giustini%2C%20C.%2C%20Menneteau%2C%20M.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Finazzi%2C%20G.%2C%20Demarsy%2C%20E.%2C%20Ulm%2C%20R.%2C%20%26amp%3B%20Allorent%2C%20G.%20%282025%29.%20Photoreceptor-induced%20LHL4%20protects%20the%20photosystem%20II%20monomer%20in%20Chlamydomonas%20reinhardtii.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E122%3C%5C%2Fi%3E%287%29%2C%20e2418687122.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2418687122%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2418687122%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Photoreceptor-induced%20LHL4%20protects%20the%20photosystem%20II%20monomer%20in%20Chlamydomonas%20reinhardtii%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Dannay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chlo%5Cu00e9%22%2C%22lastName%22%3A%22Bertin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%22%2C%22lastName%22%3A%22Cavallari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22Albanese%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dimitri%22%2C%22lastName%22%3A%22Tolleter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9cile%22%2C%22lastName%22%3A%22Giustini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathilde%22%2C%22lastName%22%3A%22Menneteau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giovanni%22%2C%22lastName%22%3A%22Finazzi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emilie%22%2C%22lastName%22%3A%22Demarsy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roman%22%2C%22lastName%22%3A%22Ulm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Allorent%22%7D%5D%2C%22abstractNote%22%3A%22Photosynthesis%2C%20the%20fundamental%20process%20using%20light%20energy%20to%20convert%20carbon%20dioxide%20to%20organic%20matter%2C%20is%20vital%20for%20life%20on%20Earth.%20It%20relies%20on%20capturing%20light%20through%20light-harvesting%20complexes%20%28LHC%29%20in%20photosystem%20I%20%28PSI%29%20and%20PSII%20and%20on%20the%20conversion%20of%20light%20energy%20into%20chemical%20energy.%20Composition%20and%20organization%20of%20PSI%20and%20PSII%20core%20complexes%20are%20well%20conserved%20across%20evolution.%20PSII%20is%20particularly%20sensitive%20to%20photodamage%20but%20benefits%20from%20a%20large%20diversity%20of%20photoprotective%20mechanisms%2C%20finely%20tuned%20to%20handle%20the%20dynamic%20and%20ever-changing%20light%20conditions.%20Light%20Harvesting%20Complex%20protein%20family%20members%20%28LHC%20and%20LHC-like%20families%29%20have%20acquired%20a%20dual%20function%20during%20evolution.%20Members%20of%20the%20LHC%20antenna%20complexes%20of%20PS%20capture%20light%20energy%2C%20whereas%20others%20dissipate%20excess%20energy%20that%20cannot%20be%20harnessed%20for%20photosynthesis.%20This%20process%20mainly%20occurs%20through%20nonphotochemical%20quenching%20%28NPQ%29.%20In%20this%20work%2C%20we%20focus%20on%20the%20Light%20Harvesting%20complex-Like%204%20%28LHL4%29%20protein%2C%20a%20LHC-like%20protein%20induced%20by%20ultraviolet-B%20%28UV-B%29%20and%20blue%20light%20through%20UV%20Resistance%20locus%208%20%28UVR8%29%20and%20phototropin%20photoreceptor-activated%20signaling%20pathways%20in%20the%20model%20green%20microalgae%20Chlamydomonas%20reinhardtii.%20We%20demonstrate%20that%20alongside%20established%20NPQ%20effectors%2C%20LHL4%20plays%20a%20key%20role%20in%20photoprotection%2C%20preventing%20singlet%20oxygen%20accumulation%20in%20PSII%20and%20promoting%20cell%20survival%20upon%20light%20stress.%20LHL4%20protective%20function%20is%20distinct%20from%20that%20of%20NPQ-related%20proteins%2C%20as%20LHL4%20specifically%20and%20uniquely%20binds%20to%20the%20transient%20monomeric%20form%20of%20the%20core%20PSII%20complex%2C%20safeguarding%20its%20integrity.%20LHL4%20characterization%20expands%20our%20understanding%20of%20the%20interplay%20between%20light%20harvesting%20and%20photoprotection%20mechanisms%20upon%20light%20stress%20in%20photosynthetic%20microalgae.%22%2C%22date%22%3A%222025-02-18%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2418687122%22%2C%22ISSN%22%3A%221091-6490%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-02-14T07%3A29%3A11Z%22%7D%7D%2C%7B%22key%22%3A%227LBLELWK%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Etourneau%20et%20al.%22%2C%22parsedDate%22%3A%222025-03-22%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEtourneau%2C%20L.%2C%20Fancello%2C%20L.%2C%20Wieczorek%2C%20S.%2C%20Varoquaux%2C%20N.%2C%20%26amp%3B%20Burger%2C%20T.%20%282025%29.%20Penalized%20likelihood%20optimization%20for%20censored%20missing%20value%20imputation%20in%20proteomics.%20%3Ci%3EBiostatistics%20%28Oxford%2C%20England%29%3C%5C%2Fi%3E%2C%20%3Ci%3E26%3C%5C%2Fi%3E%281%29%2C%20kxaf006.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbiostatistics%5C%2Fkxaf006%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbiostatistics%5C%2Fkxaf006%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Penalized%20likelihood%20optimization%20for%20censored%20missing%20value%20imputation%20in%20proteomics%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucas%22%2C%22lastName%22%3A%22Etourneau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Fancello%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samuel%22%2C%22lastName%22%3A%22Wieczorek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nelle%22%2C%22lastName%22%3A%22Varoquaux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Burger%22%7D%5D%2C%22abstractNote%22%3A%22Label-free%20bottom-up%20proteomics%20using%20mass%20spectrometry%20and%20liquid%20chromatography%20has%20long%20been%20established%20as%20one%20of%20the%20most%20popular%20high-throughput%20analysis%20workflows%20for%20proteome%20characterization.%20However%2C%20it%20produces%20data%20hindered%20by%20complex%20and%20heterogeneous%20missing%20values%2C%20which%20imputation%20has%20long%20remained%20problematic.%20To%20cope%20with%20this%2C%20we%20introduce%20Pirat%2C%20an%20algorithm%20that%20harnesses%20this%20challenge%20using%20an%20original%20likelihood%20maximization%20strategy.%20Notably%2C%20it%20models%20the%20instrument%20limit%20by%20learning%20a%20global%20censoring%20mechanism%20from%20the%20data%20available.%20Moreover%2C%20it%20estimates%20the%20covariance%20matrix%20between%20enzymatic%20cleavage%20products%20%28ie%20peptides%20or%20precursor%20ions%29%2C%20while%20offering%20a%20natural%20way%20to%20integrate%20complementary%20transcriptomic%20information%20when%20multi-omic%20assays%20are%20available.%20Our%20benchmarking%20on%20several%20datasets%20covering%20a%20variety%20of%20experimental%20designs%20%28number%20of%20samples%2C%20acquisition%20mode%2C%20missingness%20patterns%2C%20etc.%29%20and%20using%20a%20variety%20of%20metrics%20%28differential%20analysis%20ground%20truth%20or%20imputation%20errors%29%20shows%20that%20Pirat%20outperforms%20all%20pre-existing%20imputation%20methods.%20Beyond%20the%20interest%20of%20Pirat%20as%20an%20imputation%20tool%2C%20these%20results%20pinpoint%20the%20need%20for%20a%20paradigm%20change%20in%20proteomics%20imputation%2C%20as%20most%20pre-existing%20strategies%20could%20be%20boosted%20by%20incorporating%20similar%20models%20to%20account%20for%20the%20instrument%20censorship%20or%20for%20the%20correlation%20structures%2C%20either%20grounded%20to%20the%20analytical%20pipeline%20or%20arising%20from%20a%20multi-omic%20approach.%22%2C%22date%22%3A%222025-03-22%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fbiostatistics%5C%2Fkxaf006%22%2C%22ISSN%22%3A%221468-4357%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-06-06T06%3A51%3A58Z%22%7D%7D%2C%7B%22key%22%3A%22RT2FI58V%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Hijazi%20et%20al.%22%2C%22parsedDate%22%3A%222025-03-31%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHijazi%2C%20H.%2C%20Manessier%2C%20J.%2C%20Brugiere%2C%20S.%2C%20Ravnsborg%2C%20T.%2C%20Cour%26%23xE7%3Bon%2C%20M.%2C%20Brule%2C%20B.%2C%20Merienne%2C%20K.%2C%20Jensen%2C%20O.%20N.%2C%20Hesse%2C%20A.-M.%2C%20Bruley%2C%20C.%2C%20%26amp%3B%20Pflieger%2C%20D.%20%282025%29.%20Mind%20Your%20Spectra%3A%20Points%20to%20be%20Aware%20of%20When%20Validating%20the%20Identification%20of%20Isobaric%20Histone%20Peptidoforms.%20%3Ci%3EJournal%20of%20Proteome%20Research%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jproteome.4c01056%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jproteome.4c01056%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mind%20Your%20Spectra%3A%20Points%20to%20be%20Aware%20of%20When%20Validating%20the%20Identification%20of%20Isobaric%20Histone%20Peptidoforms%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hassan%22%2C%22lastName%22%3A%22Hijazi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julie%22%2C%22lastName%22%3A%22Manessier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugiere%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tina%22%2C%22lastName%22%3A%22Ravnsborg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Cour%5Cu00e7on%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Baptiste%22%2C%22lastName%22%3A%22Brule%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karine%22%2C%22lastName%22%3A%22Merienne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ole%20N.%22%2C%22lastName%22%3A%22Jensen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Bruley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Pflieger%22%7D%5D%2C%22abstractNote%22%3A%22Mass%20spectrometry%20has%20become%20central%20to%20identifying%20and%20quantifying%20histone%20post-translational%20modifications%20%28PTMs%29%2C%20surpassing%20limitations%20of%20antibody-based%20methods.%20Histones%20are%20dynamically%20modified%20by%20multiple%20structures%2C%20especially%20at%20lysine%20residues%20on%20their%20N-terminal%20tails%2C%20to%20regulate%20DNA-templated%20processes.%20Reliable%20identification%20of%20histone%20PTMs%20remains%20challenging%20and%20still%20requires%20manual%20curation.%20This%20study%20focused%20on%20the%20Lys27-Arg40%20stretch%20of%20histone%20H3%2C%20considered%20four%20sequence%20variants%2C%20an%20increasing%20number%20of%20lysine%20PTMs%20and%20artifacts%20coming%20from%20histone%20sample%20processing%2C%20which%20resulted%20in%20many%20isobaric%20peptides.%20Our%20analysis%20revealed%20the%20value%20of%20low-mass%20b1%20and%20cyclic%20immonium%20fragment%20ions%20to%20validate%20identification%20of%20the%20distinct%20peptidoforms.%20We%20examined%20how%20MS%5C%2FMS%20spectra%20are%20transformed%20by%20common%20identification%20software%20during%20the%20conversion%20of%20raw%20files%20into%20peak%20lists%2C%20and%20highlighted%20how%20some%20parameters%20may%20erase%20the%20informative%20low-mass%20fragments.%20We%20targeted%20the%20detection%20of%2040%20H3%20K27-R40%20variant%20%5Cu00d7%20PTM%20combinations%2C%20including%20the%20mouse-specific%20variants%20H3mm7%20and%20H3mm13%2C%20in%20histone%20samples%20extracted%20from%20mouse%20testis%20and%20brain%20via%20a%20parallel%20reaction%20monitoring%20analysis.%20We%20only%20detected%20very%20low%20levels%20of%20unmodified%20H3mm7.%20Our%20work%20contributes%20to%20reliably%20deciphering%20the%20histone%20code%20shaped%20by%20distinct%20sequence%20variants%20and%20numerous%20combinations%20of%20PTMs.%22%2C%22date%22%3A%222025-03-31%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.jproteome.4c01056%22%2C%22ISSN%22%3A%221535-3907%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-06-06T06%3A44%3A30Z%22%7D%7D%2C%7B%22key%22%3A%2292Y9CIE2%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pachano%20et%20al.%22%2C%22parsedDate%22%3A%222025-04-11%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPachano%2C%20B.%2C%20Farhat%2C%20D.%20C.%2C%20Shahinas%2C%20M.%2C%20von%20Velsen%2C%20J.%2C%20Corrao%2C%20C.%2C%20Belmudes%2C%20L.%2C%20de%20Bock%2C%20P.-J.%2C%20Mas%2C%20C.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Bowler%2C%20M.%20W.%2C%20Bougdour%2C%20A.%2C%20Swale%2C%20C.%2C%20%26amp%3B%20Hakimi%2C%20M.-A.%20%282025%29.%20An%20ISWI-related%20chromatin%20remodeller%20regulates%20stage-specific%20gene%20expression%20in%20Toxoplasma%20gondii.%20%3Ci%3ENature%20Microbiology%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41564-025-01980-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41564-025-01980-2%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22An%20ISWI-related%20chromatin%20remodeller%20regulates%20stage-specific%20gene%20expression%20in%20Toxoplasma%20gondii%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Belen%22%2C%22lastName%22%3A%22Pachano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dayana%20C.%22%2C%22lastName%22%3A%22Farhat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martina%22%2C%22lastName%22%3A%22Shahinas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jill%22%2C%22lastName%22%3A%22von%20Velsen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Corrao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pieter-Jan%22%2C%22lastName%22%3A%22de%20Bock%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Mas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%20W.%22%2C%22lastName%22%3A%22Bowler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%22%2C%22lastName%22%3A%22Bougdour%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Swale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohamed-Ali%22%2C%22lastName%22%3A%22Hakimi%22%7D%5D%2C%22abstractNote%22%3A%22ATP-dependent%20chromatin%20remodellers%20are%20specialized%20multiprotein%20machines%20that%20organize%20the%20genome%20in%20eukaryotic%20cells%20and%20regulate%20its%20accessibility%20by%20repositioning%2C%20ejecting%20or%20modifying%20nucleosomes.%20However%2C%20their%20role%20in%20Toxoplasma%20gondii%20is%20poorly%20understood.%20Here%20we%20show%20that%20T.%5Cu2009gondii%20has%20evolved%20two%20divergent%20proteins%20within%20the%20imitation%20switch%20%28ISWI%29%20family%3A%20TgSNF2h%20and%20TgSNF2L.%20TgSNF2h%20specifically%20forms%20a%20core%20complex%20with%20the%20transcription%20factor%20AP2VIII-2%20and%20the%20scaffold%20protein%20TgRFTS.%20Depletion%20of%20TgRFTS%20phenocopies%20the%20knockdown%20of%20TgSNF2h%2C%20restricting%20access%20to%20chromatin%20and%20altering%20local%20gene%20expression.%20At%20the%20genomic%20level%2C%20TgSNF2h%20insulates%20highly%20transcribed%20genes%20from%20silenced%20neighbours%2C%20ensuring%20stage-specific%20gene%20regulation.%20By%20modulating%20chromatin%20accessibility%20to%20transcription%20factors%2C%20TgSNF2h%20exerts%20epistatic%20control%20over%20MORC%2C%20a%20key%20regulator%20of%20sexual%20commitment.%20Our%20findings%20show%20that%20a%20specific%20ISWI%20complex%20orchestrates%20the%20partitioning%20of%20developmental%20genes%20and%20ensures%20transcriptional%20fidelity%20throughout%20the%20parasite%20life%20cycle.%22%2C%22date%22%3A%222025-04-11%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41564-025-01980-2%22%2C%22ISSN%22%3A%222058-5276%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-04-14T06%3A39%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22IBQF8IWZ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Brunchault%20et%20al.%22%2C%22parsedDate%22%3A%222025-04-24%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBrunchault%2C%20M.%20R.%2C%20Hesse%2C%20A.-M.%2C%20Schaeffer%2C%20J.%2C%20Fr%26%23xF6%3Bhlich%2C%20A.%2C%20Saintpierre%2C%20A.%2C%20Decourt%2C%20C.%2C%20Combes%2C%20F.%2C%20Nawabi%2C%20H.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20%26amp%3B%20Belin%2C%20S.%20%282025%29.%20Proteomics-based%20characterization%20of%20ribosome%20heterogeneity%20in%20adult%20mouse%20organs.%20%3Ci%3ECellular%20and%20Molecular%20Life%20Sciences%3A%20CMLS%3C%5C%2Fi%3E%2C%20%3Ci%3E82%3C%5C%2Fi%3E%281%29%2C%20175.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00018-025-05708-7%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00018-025-05708-7%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Proteomics-based%20characterization%20of%20ribosome%20heterogeneity%20in%20adult%20mouse%20organs%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%20R.%22%2C%22lastName%22%3A%22Brunchault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Schaeffer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Albrecht%22%2C%22lastName%22%3A%22Fr%5Cu00f6hlich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ana%22%2C%22lastName%22%3A%22Saintpierre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Decourt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Combes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Homaira%22%2C%22lastName%22%3A%22Nawabi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephane%22%2C%22lastName%22%3A%22Belin%22%7D%5D%2C%22abstractNote%22%3A%22The%20translation%20process%2C%20leading%20to%20protein%20synthesis%20from%20mRNA%2C%20has%20been%20long%20thought%20to%20be%20invariable%20in%20all%20cellular%20organisms.%20Increasing%20evidence%20shows%20that%20it%20is%20finely%20regulated%20by%20variable%20features%20of%20the%20translation%20machinery.%20Notably%2C%20ribosomes%2C%20the%20functional%20units%20of%20protein%20synthesis%2C%20are%20suggested%20to%20display%20variations%20in%20their%20composition%2C%20depending%20on%20the%20developmental%20stage%2C%20cell%20type%20or%20physio-pathological%20context%2C%20thus%20hinting%20a%20new%20level%20of%20actionable%20regulation%20of%20gene%20expression.%20Yet%2C%20a%20comprehensive%20map%20of%20the%20heterogeneity%20of%20ribosome%20composition%20in%20ribosomal%20proteins%20%28RPs%29%20in%20different%20organs%20and%20tissues%20is%20not%20available.%20In%20this%20work%2C%20we%20explored%20tissue-specific%20ribosome%20heterogeneity%20using%20mass%20spectrometry-based%20quantitative%20proteomic%20characterization%20of%20ribosomal%20fractions%20purified%20from%2014%20adult%20mouse%20organs%20and%20tissues.%20We%20performed%20crossed%20clustering%20and%20statistical%20analyses%20of%20RP%20composition%20to%20highlight%20stable%2C%20variable%20and%20tissue-specific%20RPs%20across%20organs%20and%20tissues.%20Focusing%20on%20specific%20RPs%2C%20we%20validated%20their%20varying%20abundances%20using%20a%20targeted%20proteomic%20approach%20and%20western%20blot%20analyses%2C%20providing%20further%20insights%20into%20the%20tissue-specific%20ribosome%20RP%20signature.%20Finally%2C%20we%20investigated%20the%20origin%20of%20RP%20variations%20in%20ribosome%20fraction%20of%20the%20different%20tissues%2C%20by%20comparing%20RP%20relative%20amounts%20in%20our%20ribosomal%20proteomic%20dataset%20with%20their%20corresponding%20transcript%20abundances%20in%20three%20independent%20transcriptomic%20datasets.%20Interestingly%2C%20we%20found%20that%2C%20in%20some%20tissues%2C%20the%20RP%20abundance%20in%20purified%20ribosomes%20does%20not%20always%20correlate%20with%20the%20corresponding%20RP%20transcript%20level%2C%20arguing%20for%20a%20translational%20regulation%20of%20RP%20expression%2C%20and%5C%2For%20a%20regulated%20incorporation%20of%20RPs%20into%20ribosomes.%20Altogether%2C%20our%20data%20support%20the%20notion%20of%20a%20tissue-specific%20RP%20signature%20of%20ribosomes%2C%20which%20opens%20avenues%20to%20study%20how%20specific%20ribosomal%20composition%20provides%20an%20additional%20level%20of%20regulation%20to%20control%20gene%20expression%20in%20different%20tissues%20and%20organs.%22%2C%22date%22%3A%222025-04-24%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00018-025-05708-7%22%2C%22ISSN%22%3A%221420-9071%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-06-10T14%3A12%3A47Z%22%7D%7D%2C%7B%22key%22%3A%223998LI7M%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Robert%20et%20al.%22%2C%22parsedDate%22%3A%222025-05-19%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERobert%2C%20M.%20G.%2C%20Swale%2C%20C.%2C%20Pachano%2C%20B.%2C%20D%26%23xE9%3Bp%26%23xE9%3Bry%2C%20L.%2C%20Bellini%2C%20V.%2C%20Dard%2C%20C.%2C%20Cannella%2C%20D.%2C%20Corrao%2C%20C.%2C%20Belmudes%2C%20L.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Bougdour%2C%20A.%2C%20Pelloux%2C%20H.%2C%20Chapey%2C%20E.%2C%20Wallon%2C%20M.%2C%20Brenier-Pinchart%2C%20M.-P.%2C%20%26amp%3B%20Hakimi%2C%20M.-A.%20%282025%29.%20Uncovering%20biomarkers%20for%20chronic%20toxoplasmosis%20detection%20highlights%20alternative%20pathways%20shaping%20parasite%20dormancy.%20%3Ci%3EEMBO%20Molecular%20Medicine%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs44321-025-00252-0%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs44321-025-00252-0%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Uncovering%20biomarkers%20for%20chronic%20toxoplasmosis%20detection%20highlights%20alternative%20pathways%20shaping%20parasite%20dormancy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%20G%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Swale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Belen%22%2C%22lastName%22%3A%22Pachano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L%5Cu00e9a%22%2C%22lastName%22%3A%22D%5Cu00e9p%5Cu00e9ry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valeria%22%2C%22lastName%22%3A%22Bellini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Dard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominique%22%2C%22lastName%22%3A%22Cannella%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Corrao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%22%2C%22lastName%22%3A%22Bougdour%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Herv%5Cu00e9%22%2C%22lastName%22%3A%22Pelloux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Chapey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martine%22%2C%22lastName%22%3A%22Wallon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Pierre%22%2C%22lastName%22%3A%22Brenier-Pinchart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohamed-Ali%22%2C%22lastName%22%3A%22Hakimi%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Toxoplasma%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20a%20neurotropic%20protozoan%2C%20causes%20toxoplasmosis%2C%20a%20prevalent%20zoonotic%20and%20food-borne%20infection%2C%20posing%20significant%20risks%20to%20immunocompromised%20individuals%20and%20congenital%20cases.%20The%20chronic%20phase%2C%20characterized%20by%20dormant%2C%20cyst-forming%20bradyzoites%2C%20is%20central%20to%20disease%20progression%20but%20is%20poorly%20understood%20due%20to%20the%20lack%20of%20serological%20tests%20to%20detect%20bradyzoite-specific%20antigens.%20This%20study%20identifies%20the%20bradyzoite%20serological%20marker%20%28BSM%29%20and%20cyst-associated%20BCLA%20as%20effective%20biomarkers%20for%20chronic%20toxoplasmosis.%20These%20markers%20showed%20high%20sensitivity%20and%20specificity%20in%20detecting%20cyst-bearing%20mice%20and%20had%20a%20positivity%20rate%20of%2030%25%20in%20humans%20with%20prior%20immunity.%20Bradyzoite%20serology%20helps%20to%20discriminate%20between%20recent%20and%20past%20infections%2C%20with%20BCLA%20improving%20the%20accuracy%20of%20the%20diagnosis%20of%20congenital%20infections.%20Mechanistic%20analyses%20show%20that%20the%20chromatin%20modifiers%20MORC%20and%20HDAC3%20epistatically%20regulate%20BFD1%2C%20a%20key%20bradyzoite%20regulator.%20While%20BFD1%20controls%20the%20expression%20of%20bradyzoite%20genes%20such%20as%20BCLA%2C%20a%20specific%20subset%2C%20including%20BSM%2C%20is%20regulated%20independently%20of%20BFD1.%20This%20multilayered%20regulation%20complicates%20the%20understanding%20of%20parasite%20persistence%20in%20humans%2C%20but%20offers%20promise%20for%20improved%20serologic%20diagnosis%20during%20pregnancy%2C%20but%20also%20in%20individuals%20with%20mental%20illness.%22%2C%22date%22%3A%222025-05-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs44321-025-00252-0%22%2C%22ISSN%22%3A%221757-4684%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.embopress.org%5C%2Fdoi%5C%2Ffull%5C%2F10.1038%5C%2Fs44321-025-00252-0%22%2C%22collections%22%3A%5B%22TK2D2GKR%22%2C%223B3CGQ6V%22%5D%2C%22dateModified%22%3A%222025-06-10T14%3A12%3A20Z%22%7D%7D%5D%7D
Geshkovski, V., Hijazi, H., Manessier, J., Brugière, S., Courçon, M., Vachon, G., Pflieger, D., & Carles, C. C. (2025). Quantitative Profiling of Histone Variants and Posttranslational Modifications by Tandem Mass Spectrometry in Arabidopsis. Methods in Molecular Biology (Clifton, N.J.), 2873, 19–38. https://doi.org/10.1007/978-1-0716-4228-3_2
Santini, S., Lartigue, A., Alempic, J.-M., Couté, Y., Belmudes, L., Brazelton, W. J., Lang, S. Q., Claverie, J.-M., Legendre, M., & Abergel, C. (2025). Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae. The ISME Journal, wraf002. https://doi.org/10.1093/ismejo/wraf002
Dannay, M., Bertin, C., Cavallari, E., Albanese, P., Tolleter, D., Giustini, C., Menneteau, M., Brugière, S., Couté, Y., Finazzi, G., Demarsy, E., Ulm, R., & Allorent, G. (2025). Photoreceptor-induced LHL4 protects the photosystem II monomer in Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences of the United States of America, 122(7), e2418687122. https://doi.org/10.1073/pnas.2418687122
Etourneau, L., Fancello, L., Wieczorek, S., Varoquaux, N., & Burger, T. (2025). Penalized likelihood optimization for censored missing value imputation in proteomics. Biostatistics (Oxford, England), 26(1), kxaf006. https://doi.org/10.1093/biostatistics/kxaf006
Hijazi, H., Manessier, J., Brugiere, S., Ravnsborg, T., Courçon, M., Brule, B., Merienne, K., Jensen, O. N., Hesse, A.-M., Bruley, C., & Pflieger, D. (2025). Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.4c01056
Pachano, B., Farhat, D. C., Shahinas, M., von Velsen, J., Corrao, C., Belmudes, L., de Bock, P.-J., Mas, C., Couté, Y., Bowler, M. W., Bougdour, A., Swale, C., & Hakimi, M.-A. (2025). An ISWI-related chromatin remodeller regulates stage-specific gene expression in Toxoplasma gondii. Nature Microbiology. https://doi.org/10.1038/s41564-025-01980-2
Brunchault, M. R., Hesse, A.-M., Schaeffer, J., Fröhlich, A., Saintpierre, A., Decourt, C., Combes, F., Nawabi, H., Couté, Y., & Belin, S. (2025). Proteomics-based characterization of ribosome heterogeneity in adult mouse organs. Cellular and Molecular Life Sciences: CMLS, 82(1), 175. https://doi.org/10.1007/s00018-025-05708-7
Robert, M. G., Swale, C., Pachano, B., Dépéry, L., Bellini, V., Dard, C., Cannella, D., Corrao, C., Belmudes, L., Couté, Y., Bougdour, A., Pelloux, H., Chapey, E., Wallon, M., Brenier-Pinchart, M.-P., & Hakimi, M.-A. (2025). Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy. EMBO Molecular Medicine. https://doi.org/10.1038/s44321-025-00252-0
EDyP-Service
238648
24RQUPQD
2025
1
apa
50
date
asc
1494
https://www.edyp.fr/web/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-c9f926535b1dbcb229037d4414452684%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ZB593V99%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Yuan%20et%20al.%22%2C%22parsedDate%22%3A%222025-03-15%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EYuan%2C%20Y.%2C%20Iannetta%2C%20A.%20A.%2C%20Kim%2C%20M.%2C%20Sadecki%2C%20P.%20W.%2C%20Arend%2C%20M.%2C%20Tsichla%2C%20A.%2C%20%26%23xC1%3Bguila%20Ruiz-Sola%2C%20M.%2C%20Kepesidis%2C%20G.%2C%20Falconet%2C%20D.%2C%20Thevenon%2C%20E.%2C%20Tardif%2C%20M.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Kleman%2C%20J.%20P.%2C%20Sizova%2C%20I.%2C%20Schilling%2C%20M.%2C%20Jouhet%2C%20J.%2C%20Hegemann%2C%20P.%2C%20Li-Beisson%2C%20Y.%2C%20%26%23x2026%3B%20Petroutsos%2C%20D.%20%282025%29.%20Phototropin%20connects%20blue%20light%20perception%20to%20starch%20metabolism%20in%20green%20algae.%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2C%20%3Ci%3E16%3C%5C%2Fi%3E%281%29%2C%202545.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-025-57809-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-025-57809-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Phototropin%20connects%20blue%20light%20perception%20to%20starch%20metabolism%20in%20green%20algae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yizhong%22%2C%22lastName%22%3A%22Yuan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%20A.%22%2C%22lastName%22%3A%22Iannetta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Minjae%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patric%20W.%22%2C%22lastName%22%3A%22Sadecki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marius%22%2C%22lastName%22%3A%22Arend%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Angeliki%22%2C%22lastName%22%3A%22Tsichla%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22%5Cu00c1guila%20Ruiz-Sola%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Georgios%22%2C%22lastName%22%3A%22Kepesidis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denis%22%2C%22lastName%22%3A%22Falconet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuel%22%2C%22lastName%22%3A%22Thevenon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marianne%22%2C%22lastName%22%3A%22Tardif%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%20Philippe%22%2C%22lastName%22%3A%22Kleman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Irina%22%2C%22lastName%22%3A%22Sizova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Schilling%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juliette%22%2C%22lastName%22%3A%22Jouhet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Hegemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yonghua%22%2C%22lastName%22%3A%22Li-Beisson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zoran%22%2C%22lastName%22%3A%22Nikoloski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Bastien%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leslie%20M.%22%2C%22lastName%22%3A%22Hicks%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dimitris%22%2C%22lastName%22%3A%22Petroutsos%22%7D%5D%2C%22abstractNote%22%3A%22In%20photosynthetic%20organisms%2C%20light%20acts%20as%20an%20environmental%20signal%20to%20control%20their%20development%20and%20physiology%2C%20as%20well%20as%20energy%20source%20to%20drive%20the%20conversion%20of%20CO2%20into%20carbohydrates%20used%20for%20growth%20or%20storage.%20The%20main%20storage%20carbohydrate%20in%20green%20algae%20is%20starch%2C%20which%20accumulates%20during%20the%20day%20and%20is%20broken%20down%20at%20night%20to%20meet%20cellular%20energy%20demands.%20The%20signaling%20role%20of%20light%20quality%20in%20the%20regulation%20of%20starch%20accumulation%20remains%20unexplored.%20Here%2C%20we%20identify%20PHOTOTROPIN-MEDIATED%20SIGNALING%20KINASE%201%20%28PMSK1%29%20as%20a%20key%20regulator%20of%20starch%20metabolism%20in%20Chlamydomonas%20reinhardtii.%20In%20its%20phosphorylated%20form%20%28PMSK1-P%29%2C%20it%20activates%20GLYCERALDEHYDE-3-PHOSPHATE%20DEHYDROGENASE%20%28GAP1%29%2C%20promoting%20starch%20biosynthesis.%20We%20show%20that%20blue%20light%2C%20perceived%20by%20PHOTOTROPIN%2C%20induces%20PMSK1%20dephosphorylation%20that%20in%20turn%20represses%20GAP1%20mRNA%20levels%20and%20reduces%20starch%20accumulation.%20These%20findings%20reveal%20a%20previously%20uncharacterized%20blue%20light-mediated%20signaling%20pathway%20that%20advances%20our%20understanding%20of%20photoreceptor-controlled%20carbon%20metabolism%20in%20microalgae.%22%2C%22date%22%3A%222025-03-15%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-025-57809-3%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%2224RQUPQD%22%5D%2C%22dateModified%22%3A%222025-03-17T07%3A47%3A03Z%22%7D%7D%2C%7B%22key%22%3A%22WI5BVTA9%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Rodrigues%20Oliveira%20et%20al.%22%2C%22parsedDate%22%3A%222025-04-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERodrigues%20Oliveira%2C%20A.%2C%20Chevalier%2C%20C.%2C%20Wargny%2C%20M.%2C%20Pakulska%2C%20V.%2C%20Caradeuc%2C%20C.%2C%20Cloteau%2C%20C.%2C%20Letertre%2C%20M.%20P.%20M.%2C%20Giraud%2C%20N.%2C%20Bertho%2C%20G.%2C%20Bigot-Corbel%2C%20E.%2C%20Carpentier%2C%20M.%2C%20Nouadje%2C%20G.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Le%20May%2C%20C.%2C%20Cariou%2C%20B.%2C%20Hadjadj%2C%20S.%2C%20%26amp%3B%20Croyal%2C%20M.%20%282025%29.%20Methylglyoxal-Induced%20Glycation%20of%20Plasma%20Albumin%3A%20From%20Biomarker%20Discovery%20to%20Clinical%20Use%20for%20Prediction%20of%20New-Onset%20Diabetes%20in%20Individuals%20with%20Prediabetes.%20%3Ci%3EClinical%20Chemistry%3C%5C%2Fi%3E%2C%20hvaf035.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fclinchem%5C%2Fhvaf035%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fclinchem%5C%2Fhvaf035%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Methylglyoxal-Induced%20Glycation%20of%20Plasma%20Albumin%3A%20From%20Biomarker%20Discovery%20to%20Clinical%20Use%20for%20Prediction%20of%20New-Onset%20Diabetes%20in%20Individuals%20with%20Prediabetes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ars%5Cu00eanio%22%2C%22lastName%22%3A%22Rodrigues%20Oliveira%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chlo%5Cu00e9%22%2C%22lastName%22%3A%22Chevalier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthieu%22%2C%22lastName%22%3A%22Wargny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Victoria%22%2C%22lastName%22%3A%22Pakulska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9dric%22%2C%22lastName%22%3A%22Caradeuc%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chlo%5Cu00e9%22%2C%22lastName%22%3A%22Cloteau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marine%20P.%20M.%22%2C%22lastName%22%3A%22Letertre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolas%22%2C%22lastName%22%3A%22Giraud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gildas%22%2C%22lastName%22%3A%22Bertho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edith%22%2C%22lastName%22%3A%22Bigot-Corbel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maxime%22%2C%22lastName%22%3A%22Carpentier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Georges%22%2C%22lastName%22%3A%22Nouadje%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9dric%22%2C%22lastName%22%3A%22Le%20May%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertrand%22%2C%22lastName%22%3A%22Cariou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samy%22%2C%22lastName%22%3A%22Hadjadj%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mika%5Cu00ebl%22%2C%22lastName%22%3A%22Croyal%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Methylglyoxal%20%28MGO%29%20is%20a%20potent%20glycating%20agent%20that%20contributes%20to%20the%20pathogenesis%20of%20diabetes.%20However%2C%20MGO%20is%20unstable%20in%20plasma%20without%20demanding%20sample%20preparation%20at%20blood%20collection%2C%20limiting%20its%20clinical%20utility%20as%20a%20biomarker.%20We%20aimed%20to%20discover%20reliable%20MGO-glycated%20albumin%20%28ALB%29-derived%20biomarkers%20and%20to%20assess%20their%20association%20with%20new-onset%20diabetes%20%28NOD%29%20in%20people%20with%20prediabetes.%5CnMETHODS%3A%20Bottom-up%20mass%20spectrometry-based%20proteomics%20was%20used%20to%20discover%20peptide%20biomarkers%20of%20MGO-glycated%20ALB%2C%20including%20MGO-derived%20hydroimidazolone%20%28MGH%29-ALB219-225%2C%20which%20proved%20to%20be%20biologically%20stable%20and%20reliable%20for%20large-scale%20analyses%20in%20human%20plasma.%20After%20assay%20validation%2C%20the%20IT-DIAB%20%28Innovation%20Th%5Cu00e9rapeutique%20DIAB%5Cu00e8te%29%20prospective%20study%2C%20conducted%20in%20300%20individuals%20with%20impaired%20fasting%20plasma%20glucose%20%28FPG%29%20levels%20%28110%20to%20125%5Cu2005mg%5C%2FdL%2C%206.1%20to%206.9%5Cu2005mmol%5C%2FL%29%2C%20was%20used%20to%20assess%20the%20association%20between%20plasma%20MGH-ALB219-225%20and%20NOD%2C%20defined%20as%20FPG%20%5Cu2265126%5Cu2005mg%5C%2FdL%20%287%5Cu2005mmol%5C%2FL%29%2C%20using%20Kaplan-Meier%20curves%20and%20Cox%20models.%5CnRESULTS%3A%20In%20total%2C%20113%20participants%20of%20the%20IT-DIAB%20study%20developed%20NOD%20during%20a%20median%20follow-up%20of%205%20years.%20There%20was%20a%20graded%20association%20between%20the%20baseline%20plasma%20MGH-ALB219-225%20concentration%20and%20incident%20NOD%20%28log-rank%20P%20%3C%200.0001%29%2C%20in%20contrast%20to%20a%20lack%20of%20association%20for%20plasma%20MGO%20and%20total%20or%20glycated%20ALB%20%28commercial%20kit%29.%20After%20adjustment%20for%20age%2C%20sex%2C%20body%20mass%20index%2C%20FPG%2C%20hemoglobin%20%28Hb%29%20A1c%2C%20and%20ALB%2C%20the%20plasma%20levels%20of%20MGH-ALB219-225%20were%20associated%20with%20NOD%20%28hazard%20ratio%20%5BHR%5D%20per%20one%20SD%20%5B95%25%20CI%5D%20%3D%201.50%20%5B1.26-1.78%5D%3B%20P%20%3C%200.0001%29.%5CnCONCLUSIONS%3A%20MGH-ALB219-225%20is%20a%20novel%20and%20stable%20peptide%20biomarker%20of%20MGO-glycated%20ALB%2C%20whose%20plasma%20levels%20are%20positively%20associated%20with%20an%20increased%20risk%20of%20NOD%20in%20individuals%20with%20prediabetes%2C%20independently%20of%20traditional%20risk%20factors.%20ClinicalTrials.gov%20Registration%20Number%3A%20NCT01218061.%22%2C%22date%22%3A%222025-04-07%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fclinchem%5C%2Fhvaf035%22%2C%22ISSN%22%3A%221530-8561%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%2C%2224RQUPQD%22%5D%2C%22dateModified%22%3A%222025-04-08T06%3A25%3A03Z%22%7D%7D%2C%7B%22key%22%3A%22CIJ5DAYE%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Monge%5Cu2010Waleryszak%20et%20al.%22%2C%22parsedDate%22%3A%222025-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMonge%26%23x2010%3BWaleryszak%2C%20L.%2C%20Girard%2C%20M.%2C%20Carcagno%2C%20M.%2C%20Culerrier%2C%20R.%2C%20Vic%26%23xE9%3Bdo%2C%20C.%2C%20Martinez%2C%20Y.%2C%20V%26%23xE9%3Brin%2C%20C.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Pacquit%2C%20V.%2C%20%26amp%3B%20Deslandes%2C%20L.%20%282025%29.%20Three%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EARID%3C%5C%2Fspan%3E%20proteins%20involved%20in%20chromatin%20remodeling%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EPEAT%3C%5C%2Fspan%3E%20complexes%20are%20targeted%20by%20the%20%3Ci%3ERalstonia%20solanacearum%3C%5C%2Fi%3E%20effector%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EPopP2%3C%5C%2Fspan%3E%20and%20contribute%20to%20bacterial%20wilt%20disease.%20%3Ci%3EThe%20Plant%20Journal%3C%5C%2Fi%3E%2C%20%3Ci%3E122%3C%5C%2Fi%3E%283%29%2C%20e70205.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Ftpj.70205%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Ftpj.70205%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Three%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EARID%3C%5C%2Fspan%3E%20proteins%20involved%20in%20chromatin%20remodeling%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EPEAT%3C%5C%2Fspan%3E%20complexes%20are%20targeted%20by%20the%20%3Ci%3ERalstonia%20solanacearum%3C%5C%2Fi%3E%20effector%20%3Cspan%20style%3D%5C%22font-variant%3Asmall-caps%3B%5C%22%3EPopP2%3C%5C%2Fspan%3E%20and%20contribute%20to%20bacterial%20wilt%20disease%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L%5Cu00e9a%22%2C%22lastName%22%3A%22Monge%5Cu2010Waleryszak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maxime%22%2C%22lastName%22%3A%22Girard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00e9lanie%22%2C%22lastName%22%3A%22Carcagno%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Culerrier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Vic%5Cu00e9do%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Martinez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22V%5Cu00e9rin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Val%5Cu00e9rie%22%2C%22lastName%22%3A%22Pacquit%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laurent%22%2C%22lastName%22%3A%22Deslandes%22%7D%5D%2C%22abstractNote%22%3A%22SUMMARY%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Like%20many%20gram%5Cu2010negative%20phytopathogenic%20bacteria%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Ralstonia%20solanacearum%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20uses%20a%20type%20III%20secretion%20system%20to%20deliver%20into%20host%20cells%20a%20cocktail%20of%20effector%20proteins%20that%20can%20interfere%20with%20plant%20defenses%20and%20promote%20infection.%20One%20of%20these%20effectors%2C%20the%20nuclear%5Cu2010targeted%20PopP2%20acetyltransferase%2C%20was%20reported%20to%20inhibit%20many%20defensive%20WRKY%20transcription%20factors%20through%20acetylation.%20To%20gain%20a%20better%20understanding%20of%20the%20mechanisms%20by%20which%20PopP2%20might%20exert%20its%20virulence%20functions%2C%20we%20searched%20for%20other%20PopP2%5Cu2010interacting%20partners.%20Here%20we%20report%20the%20identification%20of%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Arabidopsis%20thaliana%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20AT%5Cu2010Rich%20Interaction%20Domain%20protein%203%20%28ARID3%29%20and%20its%20close%20homologs%2C%20ARID2%20and%20ARID4%2C%20as%20additional%20targets%20of%20PopP2.%20These%20ARID%20proteins%20are%20core%20components%20of%20the%20chromatin%20remodeling%20PEAT%20complexes%20that%20regulate%20gene%20expression%20through%20histone%20%28de%29acetylation%20and%20deubiquitination.%20In%20yeast%2C%20PopP2%20binds%20the%20conserved%20C%5Cu2010terminal%20part%20of%20ARID2%5C%2F3%5C%2F4%2C%20which%20contains%20an%20%5Cu03b1%5Cu2010crystallin%20domain%20putatively%20involved%20in%20their%20homo%5Cu2010oligomerization.%20ARID2%5C%2F3%5C%2F4%20behave%20as%20substrates%20of%20PopP2%20acetyltransferase%20activity%2C%20which%20causes%20the%20acetylation%20of%20several%20lysine%20residues%20conserved%20between%20these%20three%20proteins%20and%20located%20near%20their%20%5Cu03b1%5Cu2010crystallin%20domain.%20Interestingly%2C%20while%20PopP2%20negatively%20affects%20ARID3%20and%20ARID4%20self%5Cu2010interactions%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20in%20planta%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20it%20promotes%20the%20interaction%20of%20ARID3%20and%20ARID4%20with%20PWWP1%2C%20another%20component%20of%20PEAT%20complexes%2C%20with%20which%20PopP2%20can%20also%20interact.%20This%20study%20also%20reveals%20that%20disruption%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20ARID2%5C%2F3%5C%2F4%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20results%20in%20reduced%20growth%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20R.%20solanacearum%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20Overall%2C%20our%20data%20are%20consistent%20with%20a%20model%20in%20which%20PopP2%20targets%20several%20components%20of%20PEAT%20complexes%20to%20interfere%20with%20their%20epigenetic%20regulatory%20functions%20and%20promote%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Ralstonia%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20infection%20in%20Arabidopsis.%22%2C%22date%22%3A%222025-05%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1111%5C%2Ftpj.70205%22%2C%22ISSN%22%3A%220960-7412%2C%201365-313X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1111%5C%2Ftpj.70205%22%2C%22collections%22%3A%5B%2224RQUPQD%22%5D%2C%22dateModified%22%3A%222025-05-13T14%3A42%3A25Z%22%7D%7D%2C%7B%22key%22%3A%22HU9QE5S2%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Guedouari%20et%20al.%22%2C%22parsedDate%22%3A%222025-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGuedouari%2C%20H.%2C%20Dia%2C%20M.%2C%20Geoffray%2C%20J.%2C%20Brun%2C%20C.%2C%20Moulin%2C%20F.%2C%20Givre%2C%20L.%2C%20Belmudes%2C%20L.%2C%20Leon%2C%20C.%2C%20Chanon%2C%20S.%2C%20Ji-Cao%2C%20J.%2C%20Chouabe%2C%20C.%2C%20Ducreux%2C%20S.%2C%20Da%20Silva%2C%20C.%20C.%2C%20Gomez%2C%20L.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Thibault%2C%20H.%2C%20Rieusset%2C%20J.%2C%20%26amp%3B%20Paillard%2C%20M.%20%282025%29.%20Structural%20and%20functional%20characterization%20of%20the%20cardiac%20mitochondria-associated%20reticular%20membranes%20in%20the%20Ob%5C%2FOb%20mouse%20model.%20%3Ci%3EJournal%20of%20Molecular%20and%20Cellular%20Cardiology%20Plus%3C%5C%2Fi%3E%2C%20100453.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jmccpl.2025.100453%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jmccpl.2025.100453%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20and%20functional%20characterization%20of%20the%20cardiac%20mitochondria-associated%20reticular%20membranes%20in%20the%20Ob%5C%2FOb%20mouse%20model%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hala%22%2C%22lastName%22%3A%22Guedouari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maya%22%2C%22lastName%22%3A%22Dia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juliette%22%2C%22lastName%22%3A%22Geoffray%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Brun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florentin%22%2C%22lastName%22%3A%22Moulin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucas%22%2C%22lastName%22%3A%22Givre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christelle%22%2C%22lastName%22%3A%22Leon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephanie%22%2C%22lastName%22%3A%22Chanon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jingwei%22%2C%22lastName%22%3A%22Ji-Cao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Chouabe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Ducreux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%20Crola%22%2C%22lastName%22%3A%22Da%20Silva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ludovic%22%2C%22lastName%22%3A%22Gomez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helene%22%2C%22lastName%22%3A%22Thibault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Rieusset%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Melanie%22%2C%22lastName%22%3A%22Paillard%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222025-05%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jmccpl.2025.100453%22%2C%22ISSN%22%3A%2227729761%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flinkinghub.elsevier.com%5C%2Fretrieve%5C%2Fpii%5C%2FS2772976125001722%22%2C%22collections%22%3A%5B%2224RQUPQD%22%5D%2C%22dateModified%22%3A%222025-05-13T14%3A42%3A32Z%22%7D%7D%2C%7B%22key%22%3A%22EGWVBXKT%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ruel%20et%20al.%22%2C%22parsedDate%22%3A%222025-05-12%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERuel%2C%20J.%2C%20Nguyen%2C%20T.%20Q.%20N.%2C%20Morishita%2C%20Y.%2C%20Usclat%2C%20A.%2C%20Martin%2C%20L.%2C%20Amara%2C%20P.%2C%20Kieffer-Jaquinod%2C%20S.%2C%20Stefanoiu%2C%20M.%20C.%2C%20de%20la%20Mora%2C%20E.%2C%20Morinaka%2C%20B.%20I.%2C%20%26amp%3B%20Nicolet%2C%20Y.%20%282025%29.%20Peptide%20Recognition%20and%20Mechanism%20of%20the%20Radical%20S-Adenosyl-l-methionine%20Multiple%20Cyclophane%20Synthase%20ChlB.%20%3Ci%3EJournal%20of%20the%20American%20Chemical%20Society%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Fjacs.4c16004%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Fjacs.4c16004%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Peptide%20Recognition%20and%20Mechanism%20of%20the%20Radical%20S-Adenosyl-l-methionine%20Multiple%20Cyclophane%20Synthase%20ChlB%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00e9mie%22%2C%22lastName%22%3A%22Ruel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thi%20Quynh%20Ngoc%22%2C%22lastName%22%3A%22Nguyen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohei%22%2C%22lastName%22%3A%22Morishita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%22%2C%22lastName%22%3A%22Usclat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lydie%22%2C%22lastName%22%3A%22Martin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Amara%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Kieffer-Jaquinod%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20Cristina%22%2C%22lastName%22%3A%22Stefanoiu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eugenio%22%2C%22lastName%22%3A%22de%20la%20Mora%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brandon%20I.%22%2C%22lastName%22%3A%22Morinaka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yvain%22%2C%22lastName%22%3A%22Nicolet%22%7D%5D%2C%22abstractNote%22%3A%22Ribosomally%20synthesized%20and%20post-translationally%20modified%20peptides%20%28RiPPs%29%20represent%20a%20valuable%20class%20of%20natural%20products%2C%20often%20featuring%20macrocyclization%2C%20which%20enhances%20stability%20and%20rigidity%20to%20achieve%20specific%20conformations%2C%20frequently%20underlying%20antibiotic%20activity.%20ChlB%20is%20a%20metalloenzyme%20with%20two%20catalytic%20domains%5Cu2500a%20radical%20S-adenosyl-l-methionine%20%28SAM%29%20domain%20and%20an%20%5Cu03b1-ketoglutarate-dependent%20oxygenase%5Cu2500that%20work%20in%20tandem%20to%20sequentially%20form%20three%20cyclophanes%20and%20introduce%20three%20hydroxyl%20groups%20into%20its%20substrate%20peptide%2C%20ChlA.%20Here%2C%20we%20present%20the%20crystal%20structure%20of%20the%20radical%20SAM%20domain%20of%20ChlB%20in%20complex%20with%20ChlA%2C%20revealing%20the%20mechanism%20underlying%20cyclophane%20formation.%20These%20structures%20also%20elucidate%20how%20the%20leader%20sequence%20of%20ChlA%20interacts%20with%20ChlB.%20By%20combining%20structural%2C%20in%20vitro%2C%20and%20in%20vivo%20approaches%2C%20we%20determined%20the%20precise%20sequence%20of%20the%20three%20cyclophane%20formations%2C%20interspersed%20with%20hydroxylation%20events.%20Our%20findings%20demonstrate%20a%20back-and-forth%20movement%20of%20the%20core%20peptide%20between%20the%20radical%20SAM%20domain%20and%20the%20oxygenase%20domain%2C%20which%20drives%20the%20stepwise%20modification%20process%2C%20leading%20to%20the%20fully%20modified%20peptide.%22%2C%22date%22%3A%222025-05-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1021%5C%2Fjacs.4c16004%22%2C%22ISSN%22%3A%221520-5126%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%2224RQUPQD%22%5D%2C%22dateModified%22%3A%222025-05-13T10%3A38%3A37Z%22%7D%7D%5D%7D
Yuan, Y., Iannetta, A. A., Kim, M., Sadecki, P. W., Arend, M., Tsichla, A., Águila Ruiz-Sola, M., Kepesidis, G., Falconet, D., Thevenon, E., Tardif, M., Brugière, S., Couté, Y., Kleman, J. P., Sizova, I., Schilling, M., Jouhet, J., Hegemann, P., Li-Beisson, Y., … Petroutsos, D. (2025). Phototropin connects blue light perception to starch metabolism in green algae. Nature Communications, 16(1), 2545. https://doi.org/10.1038/s41467-025-57809-3
Rodrigues Oliveira, A., Chevalier, C., Wargny, M., Pakulska, V., Caradeuc, C., Cloteau, C., Letertre, M. P. M., Giraud, N., Bertho, G., Bigot-Corbel, E., Carpentier, M., Nouadje, G., Couté, Y., Le May, C., Cariou, B., Hadjadj, S., & Croyal, M. (2025). Methylglyoxal-Induced Glycation of Plasma Albumin: From Biomarker Discovery to Clinical Use for Prediction of New-Onset Diabetes in Individuals with Prediabetes. Clinical Chemistry, hvaf035. https://doi.org/10.1093/clinchem/hvaf035
Monge‐Waleryszak, L., Girard, M., Carcagno, M., Culerrier, R., Vicédo, C., Martinez, Y., Vérin, C., Couté, Y., Pacquit, V., & Deslandes, L. (2025). Three ARID proteins involved in chromatin remodeling PEAT complexes are targeted by the Ralstonia solanacearum effector PopP2 and contribute to bacterial wilt disease. The Plant Journal, 122(3), e70205. https://doi.org/10.1111/tpj.70205
Guedouari, H., Dia, M., Geoffray, J., Brun, C., Moulin, F., Givre, L., Belmudes, L., Leon, C., Chanon, S., Ji-Cao, J., Chouabe, C., Ducreux, S., Da Silva, C. C., Gomez, L., Couté, Y., Thibault, H., Rieusset, J., & Paillard, M. (2025). Structural and functional characterization of the cardiac mitochondria-associated reticular membranes in the Ob/Ob mouse model. Journal of Molecular and Cellular Cardiology Plus, 100453. https://doi.org/10.1016/j.jmccpl.2025.100453
Ruel, J., Nguyen, T. Q. N., Morishita, Y., Usclat, A., Martin, L., Amara, P., Kieffer-Jaquinod, S., Stefanoiu, M. C., de la Mora, E., Morinaka, B. I., & Nicolet, Y. (2025). Peptide Recognition and Mechanism of the Radical S-Adenosyl-l-methionine Multiple Cyclophane Synthase ChlB. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.4c16004
